摘要
目的:检测结直肠癌组织中环氧合酶-2(Cyclooxygenase-2,COX-2)和增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)的表达,分析与肿瘤临床病理特征的关系;同时检测结直肠癌患者血浆中前列腺素E2(Prostaglandin E2,PGE2)的表达。方法:采用定量逆转录聚合酶链反应方法,检测40例结直肠癌手术标本中的肿瘤及癌旁无瘤结肠组织中COX-2、PCNAmRNA转录水平,并结合临床病理指标进行分析;同时采用酶联免疫吸附实验(ELISA)检测结直肠癌术前、术后及健康对照人群血清中PGE2的表达差异。结果:肿瘤组织中COX-2和PCNA的mRNA转录水平显著高于癌旁无瘤结肠组织(P<0.01),肿瘤组织中COX-2的mRNA转录水平在不同的组织分化程度、浸润深度和有无淋巴结转移中具有统计学差异(P<0.05);PCNA的mRNA转录水平与肿瘤组织的分化程度和浸润深度有关(P<0.05),和淋巴结转移无关;且两者表达呈显著正相关(P<0.01)。结直肠癌患者术前及术后血浆中PGE2水平明显高于正常对照组(P<0.05),而且术后血浆中PGE2的表达较术前显著下降(P<0.01),但仍高于对照组(P<0.05)。结论:COX-2和PCNA在结直肠癌的恶性生物学行为中起重要作用,监测外周血中PGE2水平对早期发现结直肠癌有一定参考价值。
Objective：To investigate the expression of cyclooxygenase-2 （COX-2）and proliferation cell nuclear antigen （PCNA）in colorectal carcinoma,and to identify the correlation between these expressions with the clinical biological features. Methods：The levels of COX-2 and PCNA mRNA were detected with quantitative reverse transcription-polymerase chain reaction （QRT-PCR）in 40 pairs of samples containing tumor tissues and the non-cancerous tissues. The level of PGE2 in peripheral blood was detected by ELISA. Data of COX-2 and PCNA mRNA were statistically analyzed along with the corresponding clinicopathological features. Results：The mRNA transcription levels of COX-2 and PCNA in tumor tissues were significantly higher than that in the non-cancerous tissues （P0.01）. A significant difference was found in mRNA transcription levels for COX-2 and PCNA among patients with tumors of different degrees of invasion depth and the differentiation. The mRNA transcription level of COX-2 was significantly different between patients with and without lymph node metastasis（P0.05）,which was not found in PCNA. COX-2 mRNA expression was correlated with PCNA in colorectal cancer （P0.01）. The PGE2 levels in postoperative plasma of colorectal cancer was significantly lower than that in preoperative plasma （P0.01）,but still higher than the normal plasma （P0.05）. Conclusion：COX-2 and PCNA play important roles in carcinogenesis of colorectal cancer . Measurement of PGE2 might be helpful in the early detection and diagnosis of colorectal cancer.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第10期1467-1470,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:30772115)
关键词
环氧合酶2
前列腺素E2
结直肠癌
增殖细胞核抗原
Cyclooxygenase-2
Prostaglandin E2
Colorectal carcinoma
Proliferation cell nuclear antigen
