摘要
目的 观察SD大鼠牙乳头细胞在复合有转化生长因子β，（transformingg rowthfactor—β1，TGF—β1）的微孔滤膜上形成牙本质样结构的能力，为组织工程化具有一定规则形状的牙本质提供实验依据。方法取传代培养的第二代SD大鼠牙乳头细胞消化、离心，所得细胞团与复合有TGF—β1的微孔滤膜相结合，体外培养6d或体内移植2周，观察附合物上细胞生长和硬组织形成情况。结果体外培养观察到靠近微孔滤膜的牙乳头细胞发生极化，呈高柱状沿滤膜排列，并有细胞突起伸人材料的多孔结构中，牙本质涎蛋白（dentin sialoprotein，DSP）与牙本质基质蛋白1（dentinmatrixprotein-1，DMP-1）表达阳性；体内移植2周移植物可见管状基质沿滤膜表面沉积，扫描电镜可见厚度基本一致的管状牙本质样结构，DSP、DMP-1在高柱状细胞、管状基质和邻近的牙乳头细胞中均有表达。结论复合有TGF—β1的微孔滤膜能够有效趋化和诱导成牙本质细胞前体细胞在其表面分化，并均匀分泌基质，矿化形成结构规则的牙本质样结构。
Objective To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth faetor-β1 （TGF-β1）. Methods The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass. The cell mass was seeded on the millipore filter combined with TGF-β1. The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed. Results A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein（DSP） and dentin matrix protein-1 （DMP-1） were positive in these cells. After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter. Conclusions The millipore filter combined with TGF-β1 could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2010年第11期678-683,共6页
Chinese Journal of Stomatology
基金
军队医药卫生科研项目（08G104）
关键词
牙乳头
微孔过滤器
细胞分化
牙本质再生
Dental papilla
Micropore filters
Cell differentiation
Dentin regeneration
