摘要
以甘蓝型油菜湘油13为试验材料,运用子房注射法将BT毒蛋白基因导入油菜。在授粉后第20小时至第30小时用自制的微玻针从子房中部注射0.5～1.5μg外源DNA,以高达12.8％的频率获得抗卡那霉素植株。分子杂交分析证明BT毒蛋白基因已整合到油菜基因组中,转化频率为1.6％,表明子房注射法是一种有效、实用的油菜遗传转化方法。
Using Brassica napus cv. Xiangyou 13 as materials, BT toxic protein gene was introduced into oilseed rape via ovary-injection. Anti-kanamycin plants were obtained at highest frequency of 12. 8% when 0. 5~1.5 μg foreign DNA was injected into the middle of ovary from 20th hour to 30th hour after pollination. Molecular hybrid analysis showed that BT toxic protein gene was integrated into the genome of oilseed rape, transgenic plants were obtained at frequency of 1. 6%. The transforming method of ovar-injection is efficient and practical for oilseed rape.
出处
《作物学报》
CAS
CSCD
北大核心
1999年第4期447-450,共4页
Acta Agronomica Sinica
基金
湖南省自然基金
关键词
甘蓝型油菜
遗传转化
BT毒蛋白基因
油菜
Brassica napus
Genetic transformation
BT toxic protein gene
Ovary injection
