摘要
目的 探讨L-N6-（1-亚氨乙基）赖氨酸（L-NIL）对大鼠移植肺缺血再灌注损伤的影响.方法 清洁级雄性SD大鼠18只,体重250～350 g,随机分为3组（n=6）,假手术组（S组）;肺移植组（L组）肺移植后恢复再灌注,冷缺血时间约1 h;L-NIL组再灌注即刻经尾静脉输注L-NIL 3 mg/kg.各组均于再灌注即刻经尾静脉注射0.5%伊文氏蓝0.2 ml.再灌注2 h时取左肺组织,测定肺湿/干重（W/D）比、伊文氏蓝含量、MDA含量、髓过氧化物酶（MPO）、诱导型一氧化氮合酶（iNOS）及内皮源性一氧化氮合酶（eNOS）活性;并行病理学观察.结果 与S组比较,L组肺组织W/D比和伊文氏蓝含量增加,MDA含量、MPO及iNOS活性升高,eNOS活性降低（P＜0.05）.与L组比较,L-NIL组肺组织W/D比和伊文氏蓝含量减少,MDA含量、MPO及iNOS活性降低,eNOS活性升高（P＜0.05）,肺组织毛细血管内充血减少,炎性细胞浸润减少.结论 再灌注早期静脉注射L-NIL可减轻大鼠移植肺缺血再灌注损伤.
Objective To investigate the effect of L-N6-（1-iminoethyl） Lysine（L-NIL） on ischemia-reperfusion （I/R） -induced lung injury in a rat model of lung transplantation. Methods Pathogen free male SD rats weighing 250-350g were used as donor and recipient rats in this study. The animals were randomly divided into 3groups （n = 6 each）： sham operation group （group S）; lung tratsplantation group （group L） and lung transplantation ＋ L-NIL （selective iNOS inhibitor） group （group L-NIL）. In group L and L-NIL orthotopic left lung allograft transplantation was performed. In group L-NIL 3 mg/kg was injected iv at the beginning of reperfusion. The donor lungs were removed from live donor rats and placed in Euro-collins solution at 4 ℃. The lung transplantation was performed under microscope and non-suture cuff technique was used. The implanted donor lungs were ventilated and reperfused. 0.5% Evans blue 0.2 ml was injected iv during reperfusion. The donor lungs were removed after being implanted, ventilated and reperfused for 2 h for microscopic examination and determination of iNOS, endothelial NOS （eNOS） and myeloperoxidase （MPO） activity and malondialdehyde （MDA） and Evans blue content in the lung tissue and W/D lung weight ratio. Results Lung transplantation significantly inceased W/D ratio, iNOS and MPO activity, and Evans blue and MDA content in the lung tissue and decreased eNOS activity in group L as compared with group S. L-NIL iv significantly attenuated the increase in the variables mentioned above and ameliorated capillary congestion and inflammatory cell infiltration in the lung. Conclusion Intravenous L-NIL administered at the beginning of reperfusion can reduce I/R injury to the transplanted donor lungs.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第8期973-975,共3页
Chinese Journal of Anesthesiology
基金
上海市卫生局青年基金（2007Y28）
