摘要
目的 评价瑞芬太尼预先给药对兔心肌缺血再灌注损伤的影响.方法 健康家兔30只,月龄12～18个月,体重2.5～3.5kg,随机分为3组（n=10）：缺血再灌注组（IR组）、低剂量瑞芬太尼预先给药组（R1组）和高剂量瑞芬太尼预先给药组（R2组）.3组均采用结扎左冠状动脉前降支30 min开放的方法制备心肌缺血再灌注模型,R1组和R2组分别静脉输注瑞芬太尼1.65、3.30μg·kg-1·min-130 min时进行缺血,IR组给予等容量生理盐水.于给药前（基础状态）、给药结束时、缺血30 min、再灌注6 h时采集静脉血样,测定血清心肌肌钙蛋白I（cTnI）和MB型肌酸激酶同工酶（CK-MB）的浓度.再灌注结束时处死动物,取心肌组织,光镜和电镜下观察病理学结果.结果 与IR组比较,R1组和R2组血清cTnI和CK-MB浓度降低（P＜0.01）;与R1组比较,R2组血清cTnI浓度降低（P＜0.01）.R1组和R2组心肌病理学损伤程度均轻于IR组,且R2组心肌病理学损伤程度轻于R1组.结论 瑞芬太尼预先给药可减轻兔心肌缺血再灌注损伤,且与剂量有关.
Objective To investigate the effects of remffentanil pretreatment on myocardial ischemiareperfusion （I/R） injury in rabbits. Methods Thirty healthy rabbits, aged 12-18 months, weighing 2.5-3.5 kg,were randomly divided into 3 groups （n = 10 each）： I/R group, low-dose remifentanil pretreatment group （group R1 ） and high-dose remifentanil pretreatment group （group R2 ）. Myocardial I/R was induced by ligation of anterior descending branch of left coronary artery for 30 min followed by 6 h of reperfusion in the 3 groups. Remifentanil was infused intavenously at 1.65 and 3.30 μg· kg - 1 · min- 1 for 30 min before ischemia in group R1 and R2 respectively, while equal volume of normal saline was infused instead in group I/R. Blood samples were taken for determination of serum cardiac troponin-Ⅰ （cTnI） and creatine kinase-MB isoenzyme （CK-MB） concentrations before administration （baseline）, after administration, at 30 min of ischemia and at 6 h of reperfusion. The rabbits were then sacrificed and hearts removed. Myocardial tissues were obtained for microscopic examination. Results Serum cTnI and CK-MB concentrations were significantly lower in group R1 and R2 than in group I/R and serum cTnI concentration lower in group R2 than in group R1 （ P ＜ 0.01）. Remifentanil infusion significantly attenuated the pathologic changes in a dose-dependent manner. Conclusion Remifentanil pretreatment provides protective effect against myocardial I/R injury in rabbits and it is related to the dose.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第8期943-945,共3页
Chinese Journal of Anesthesiology
基金
宁夏自然科学基金（NZ08110）
宁夏自治区卫生厅科研基金（2007）
关键词
哌啶类
心肌再灌注损伤
Piperidines
Myocardial reperfusion injury
