摘要
作为免疫化学标记物的载体，聚苯乙烯小球被广泛应用于放免药盒［１］，在这一应用中，聚苯乙烯与抗体等蛋白质之间靠非特异性物理吸附结合在一起．但这种固定化较微弱，吸附量难以控制，时间稍长后，蛋白质易于脱落而使放免药盒失效，为解决这一问题，有人曾在聚苯乙烯小...
Polystyrene spheres are widely used in radioimmumoassay kit,but it is limited by weak physical adsorption of proteins and the hard control of the amount of adsorpted proteins.The acrolein was grafted on the surface of polystyrene spheres via gamma induced pre irradiation technique.The induced aldehyde groups could react with the amino groups of proteins to form Schiff base,through which trypsin and 125 I labeled antibody were successfully immobilized on the polystyrene spheres by chemical bond.Comparing with the polystyrene blank,the acrolein grafted polystyrene spheres obviously enhanced the amount of immobilized proteins.So by using this method,the radioimmunoassay kit could be improved,but it is better to change to another monomer with aldehyde group because acrolein is irritant and easy to induce oxo addition reaction with polystyrene.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
1999年第2期252-254,共3页
Acta Polymerica Sinica
关键词
放免药盒
聚苯乙烯
丙烯醛
接枝
固定化
蛋白质
Polystyrene,Acrolein,Radiation graft polymerization,Immobilization
