摘要
染料结合法测定蛋白质在生化及临床分析中已有广泛应用，常用的染料有溴甲酚绿［１］、溴酚蓝［２］、考马斯亮蓝［３］及铬天青Ｓ［４］等，这些染料的最大吸收波长均处于可见光区．近年来，近红外染料越来越引起人们的注意［５］，因为它们的吸收及荧光光谱均处于近红外...
A new watersoluble nearIR cyanine dye was synthesized and its structure was characterized by 1H NMR, MS and IR spectra. The dye was used as a probe to establish a novel nearIR spectrophotometric method for the determination of trace amount of serum protein. The dye has a maximum absorption wavelength at 776 nm, its absorbance decreases when it reacts with protein(for example, BSA). The reaction of the dye with BSA was carried out in 002 mol/L HAcNaAc buffer system(pH 40). When 3010-6 mol/L cyanine was used, the linearity of this method was over the range of 100102190103 ng/mL by determination of HSA and globulin, and the detection limit was 50 ng/mL.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第4期555-557,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
教育部材料与生命过程分析科学开放实验室资助
