摘要
目的:研究乌骨藤的化学成分。方法:利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法及半制备高效液相色谱法,对乌骨藤的70%乙醇提取物进行分离纯化,利用核磁共振谱、质谱、红外光谱等光谱数据鉴定结构。结果:从乌骨藤中共分离鉴定了7个化合物,分别为β-谷甾醇(1),牛奶菜醇(2),二氢牛奶菜醇(3),白桦酯酸(4),羽扇豆醇(5),胡萝卜苷(6),11α-O-(2-甲基丁酰基)-12β-O-乙酰基通关藤苷元B(7)。结论:化合物4,5为首次从该属植物中分离得到。
Objective： To study the chemical constitutes of caulis Marsdeniae tenocissimae.Method： 70% ethanol extracts of caulis M.tenocissimae were isolated and purified by silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative reversed phase liquid chromatography（RPLC）.The compounds were identified on the basis of spectral analysis,including NMR,MS and IR.Result： Seven compounds were obtained and identified and their structures were identified as β-sitosterol（1）,condutirol（2）,dihydroconduritol（3）,betulinic acid（4）,lupeol（5）,daucosterol（6）,11α-О-（2-methylbutyryl）-12β-О-acetyltenacigeninB（7）.Conclusion： Compound 4,5 were isolated from the genus for the first time.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第23期3175-3177,共3页
China Journal of Chinese Materia Medica
基金
中国博士后科学基金特别项目(200902550)
辽宁省教育厅基金项目(2009T006)
大连市科技局青年基金项目(2009J22DW040)
关键词
乌骨藤
分离
结构鉴定
Marsdeniae tenocissimae
isolation
structure identification
