摘要
目的:研究一株来源于海南刺果番荔枝、能够抑制肿瘤细胞增殖的内生真菌F-31(黑团孢霉,Periconiasp.)的化学成分。方法:采用改良PDA培养基,对内生真菌F-31进行放大培养,通过大孔树脂吸附、硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等色谱手段对其发酵液及菌丝中的化学成分进行分离,利用IR,MS,NMR等多种波谱手段鉴定获得的化合物的结构,应用MTT法评价化合物的体外抗肿瘤活性。结果:从内生真菌F-31的菌液乙酸乙酯层提取部分和菌丝部分提取物中分离并鉴定了6个化合物,分别为:5-(3-hydroxybutyl)furan-2(5H)-one(1),chloranthalactone E(2),5,7-二甲氧基-6-羟基香豆素(3),(1′R,2′R,3′S,4′R)-1,2,4-三唑核酸(4),L-亮氨酸(5),L-苯丙氨酸(6)。药理活性评价结果表明该6个化合物均无明显的体外抗肿瘤活性。结论:所得的化合物中,化合物1为新化合物。
Objective： To investigate the chemical constituents of an endophytic fungus,F-31,from Annona muricata and search antitumor natural products.Method： After scaling up,the fermentation broth and mycelia were extracted by macroporous resin and chromatographied by silica gel column,Sephadex LH-20 gel column and semi-preparative HPLC.The structures of compounds were determined by the means of extensive spectroscopic data.The activity of the compounds were evaluated through MTT assay.Result： Six compounds were isolated from the fermentation broth and mycelia of this fungus,their structures were identified as 5-（3-hydroxybutyl）furan-2（5H）-one（1）,chloranthalactone E（2）,5,7-dimethyl-6-hydroxycoumarin（3）,1,2,4-triazole-（1′R,2′R,3′R,4′R）-nucleosides（4）,L-tryptophan（5）,L-phenylalanine（6）.The in vitro pharmalogical evaluation results displayed that the above compounds exhibited no inhibitory effects on the proliferation of six tumor cell lines（HCT-8,Bel-7402,BGC-823,A549,A2780 and MCF-7）.Conclusion： Among these obtained compounds,compound 1 was a new compound.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第23期3151-3155,共5页
China Journal of Chinese Materia Medica
基金
国家“十一五”重大新药创制科技专项(2009ZX09301-003-4-1)
关键词
内生真菌
化学成分
番荔枝
Endophytic fungus
chemical constituents
Annona muricata
