摘要
筛选到能催化肉桂醇生成3-苯丙醇的菌株CG10,该菌株被鉴定为毛霉菌(Mucor sp.)。对其进行生物催化研究,用气相色谱-质谱联用仪、红外光谱仪、高效液相色谱仪对产物进行了表征。通过对微生物催化反应条件的优化及对底物的耐受性实验得到最优降解条件为:糊精质量浓度为3g/L,蛋白胨质量浓度为1.6g/L,反应体系初始pH=5,肉桂醇的初始加入量为2mL/L,30℃下反应24h,二次加入量为0.5mL/L,反应8h,3次加入量为0.5mL/L,反应16h。在此条件下肉桂醇的转化率为99%,3-苯丙醇的选择性为92%,转化液中3-苯丙醇的产量较优化前提高了123%,达到2.9g/L。
A CG10 strain was isolated which could transform cinnamyl alcohol to 3-phenylpropanol.The strain was proved as Mucor sp.by bacteria identification.The biocatalyzed reaction product was characterizated by GC-MS,IR and HPLC.The optimized degradation conditions were found as 30 g/L dextrin,16 g/L peptone with the initial cinnamyl alcohol addition amount of 2 mL/L at pH 5.0 under 30 ℃ for 24 h of reaction.The secondary cinnamyl alcohol addition amount was 0.5 mL/L for 8 h of reaction,and the third cinnamyl alcohol addition amount was 0.5 mL/L for 16 h of reaction.Under the optimized conditions ,the conversion of cinnamyl alcohol was 99% ,the selectivity for 3-phenylpropanol was 92% and the yield of 3-phenylpropanol in the transformation liquid was up to 2.9 g/L,which was 123% higher than the control one.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2010年第12期2368-2372,共5页
Chemical Industry and Engineering Progress
基金
广西科学基金项目(0832002)
广西科技攻关项目(1099061-2)资助
关键词
肉桂醇
3-苯丙醇
氢化肉桂醇
生物催化
毛霉菌
cinnamyl alcohol
3-phenylpropanol
hydrocinnamyl alcohol
biocatalysis
Mucor sp.CG10
