摘要
目的:探讨载脂蛋白B(apolipoprotein B,Apo B)基因的单核苷酸多态性(single-nucleotide polymorphism,SNP)与激素性股骨头坏死中医证候的关联。方法:运用聚合酶链反应-连接酶检测技术,检测63例激素性股骨头坏死患者ApoB基因C7623T和G12619A位点的多态性,分析其基因型和等位基因频率的分布以及这两个位点SNPs与激素性股骨头坏死中医证候之间的关联。结果:ApoBC7623T的TT基因型和T等位基因在筋脉瘀滞证出现的频率高于肝肾亏损证(P<0.05);ApoBG12619A位点的SNPs与激素性股骨头坏死中医证型之间无显著性的关联(P>0.05);ApoBC7623T与G12619A位点等位基因与激素性股骨头坏死之间的连锁不平衡有显著性的关联(P<0.01)。结论:ApoBC7623T位点TT基因型和T等位基因可能与激素性股骨头坏死患者形成筋脉瘀滞证相关。
Objective:To investigate the correlation of the single -nucleotide polymorphism(SNP) of apolipoprotein B(Apo B) with Chinese medical syndrome patterns of steroid-induced avascular necrosis of the femoral head(SANFH).Methods:The polymorphism of Apo B C7623T and G12619A in 63 SANFH patients was detected with the method of polymerase chain reaction(PCR).The genotypes and the distribution of allele frequency were analyzed,and the correlation of polymorphism of Apo B C7623T and G12619A with Chinese medical syndrome patterns of SANFH was investigated.Results:TT genotype incidence and T allele frequency of Apo B C7623T were higher in SANFH patients with blood-stasis stagnated in tendons and vessels than those patients with deficiency of liver and kidney(P<0.05).The correlation between SNP of Apo B G12619A and Chinese medical syndrome patterns of SANFH was negative(P>0.05),but the allele frequency of Apo B C7623T and G12619A was positively correlated with the linkage disequilibrium of SANFH(P<0.01).Conclusion:TT genotype incidence and T allele frequency of Apo B C7623T are possibly related with blood-stasis stagnated in tendons and vessels of SANFH patients.
出处
《新中医》
CAS
北大核心
2010年第12期24-26,共3页
New Chinese Medicine
基金
山东省优秀中青年科学家科研奖励基金项目(编号:BS2009YY024)
山东省博士后创新项目(编号:200803013)
国家自然科学基金项目(编号:30672700)
