摘要
目的观察高氟染毒新西兰白兔某些氧化、抗氧化和血管功能指标的变化。方法 20只雄性新西兰白兔随机分为对照组,饮去离子水,饲基础饲料;高脂组,饮去离子水,饲基础饲料加0.5%胆固醇、7%蛋黄粉高脂饲料;高氟组,饮含氟质量浓度100mg/L高氟水,饲基础饲料;高氟高脂组,饮含氟质量浓度100mg/L高氟水,饲基础饲料加0.5%胆固醇、7%蛋黄粉高脂饲料,实验期6个月。于实验前、实验3个月、6个月取血氟离子电极法测血氟含量。实验6个月取血、心和肝制备组织匀浆,生化法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)水平;放免法检测血浆6-酮-前列腺F1α(6-keto-PGFlα)、血栓素B2(TXB2)和内皮素-1(ET-1);生化法检测血清一氧化氮合酶(NOS)、诱导型NOS(iNOS)、内皮型NOS(eNOS)活性;原位杂交法检测白细胞iNOS-mRNA和eNOS-mRNA表达。结果新西兰白兔饮用高氟水3个月、6个月时血清氟升高;6个月时血液、肝和心肌SOD、GSH-Px活性降低(P<0.01,P<0.05)、心肌MDA含量升高(P<0.05);血浆6-keto-PGFlα含量下降(P<0.01),TXB2和ET-1含量升高(P<0.01,P<0.05);血清总NOS活性下降(P<0.05),肝总NOS活力、心肌和肝iNOS活性升高(P<0.05);血白细胞iNOS-mRNA表达增强,eNOS-mRNA表达减弱(P<0.05)。析因方差分析,血清、肝和心肌SOD活性及血清MDA含量,血浆ET-1含量以及血清iNOS活性、肝总NOS活性等指标高氟与高脂具有交互增强作用(P<0.01,P<0.05)。结论高氟抑制抗氧化酶,血管内皮功能受损,机体NO代谢紊乱,此过程高氟与高脂具有一定的协同作用。
Objective To observe certain changes of oxidation,anti-oxidation and vascular function indexes of Application of New Zealand rabbit exposed by high-fluoride.Methods 20 male New Zealand rabbits were randomly divided into control group,drunk deionized water and feed basic diet.High-fat group,drunk deionized water and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet.High-fluorine group,drunk highfluoride water（ion-100mg /L） and feed basic diet.High-fluoride and high-fat group,drunk high-fluoride water（ion100mg /L） and feed basic diet plus 0.5% cholesterol and 7% egg yolk powder high fat diet.The experimental periods were 6 months.Blood samples were collected to determine the fluorine concentration in plasma,in the third and sixth month before experiment.In the sixth month of the experiment,blood,heart and liver samples were gathered to make homogenate,and detect superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） activities and malondialdehyde（MDA） contents by biochemical method.The 6-keto-prostate F1α（6-keto-PGFlα）,thromboxane B2（TXB2） and endothelin-1（ET-1） contents were detected by radioimmunoassay.Nitric oxide synthase（NOS）,inducible NOS（iNOS）,and endothelial NOS（eNOS） were detected by biochemical method.Leukocyte iNOS-mRNA and eNOS-mRNA contents were detected by situ hybridization.Results In the third and sixth month of experiment,serum fluorides were elevated in rabbits who drunk high-fluorine water,activities of SOD and GSH-Px in blood,liver and heart were decreased（P 0.01,P 0.05）,while the myocardial MDA contents increased（P 0.05）,6-keto-PGFlα contents in plasma decreased（P 0.01）,TXB2 and ET-1 levels increased（P 0.01,P 0.05）,total NOS activities in serum decreased（P 0.05）,total NOS activities in liver and iNOS activities in heart and liver increased（P 0.05）.Expression of iNOS-mRNA in leukocyte increased,expression of eNOS-mRNA decreased（P 0.05） in rabbits who drunk high-fluorine in the sixth month.Factorial analysis of variance,serum,liver and myocardial SOD activities and serum MDA contents,plasma ET-1 contents and serum iNOS activities,liver total NOS activities showed that high-fluorine and high-fat enhanced interactive（P 0.01 P 0.05）.Conclusion High-fluoride could inhibit antioxidant enzymes,impair vascular endothelial function,body NO metabolism disorder.High fluoride and high-fat could have a certain synergy in this process.
出处
《卫生研究》
CAS
CSCD
北大核心
2010年第6期751-754,共4页
Journal of Hygiene Research
基金
山东省人民政府和山东省国土资源厅资助项目(No.1212010310306)
关键词
高氟
氧化与抗氧化指标
血管损伤指标
新西兰兔
high-fluoride
oxidation and anti-oxidation index
vascular injury indicators
New Zealand rabbit
