摘要
目的探讨左旋棉酚对人鼻咽癌细胞株CNE2凋亡的影响及其机制。方法采用MTT实验检测左旋棉酚对CNE2细胞的增殖抑制作用，流式细胞术分析左旋棉酚诱导细胞凋亡及其对Bcl-2、Bax蛋白表达的影响，caspase-3分光光度法检测试剂盒测定caspase-3活性。结果≥10μmol／L左旋棉酚可显著抑制CNE2细胞增殖，并表现出剂量、时间依赖效应。左旋棉酚可调节CNE2细胞的细胞周期，使其主要被阻滞于G0／G1期，在诱导细胞凋亡过程中，凋亡相关基因Bcl-2表达下降，而Bax表达则相对上调，caspase-3活性在24h达到高峰。结论左旋棉酚在体外可诱导鼻咽癌细胞CNE2凋亡，其机制可能与Bcl-2基因下调、Bax基因上调、caspase-3的激活有关。
Objective To investigate the apoptosls-inducing effect of L-gossypol on human nasopharyngeal carcinoma cell line CNE2 and its possible mechanism. Methods The effect of L-gossypol on proliferation of CNE2 cells was estimated by MTT assay. Flow cytometry was used to analyz cell apoptosis induced by （-）-gossypol and the expression of Bcl-2,Bax proteins. Caspase-3 activity was determined by caspase-3 colorimetric assay kit. Results L- gossypol was able to inhibit the proliferation of CNE2 cells in a dose-dependent and time-dependent fashion at concen- trations more than 10 ≥10μmoL/ L.L-gossypol regulated cell cycle and G0/G1 arrest could be observed in CNE2 cells. In the process of apoptosis,the expression of Bcl-2 was down-regulated and Bax was up-regulated, caspase-3 activity was in peak at 24h. Conclusion L-gossypol could induce apoptosis in nasopharyngeal carcinoma cell line CNE2 in vitro, which may be associated with down-regulation of Bcl-2, up-regulation of Bax genes expression and caspase-3 activation.
出处
《中国基层医药》
CAS
2010年第21期2891-2893,3025,共4页
Chinese Journal of Primary Medicine and Pharmacy
关键词
左旋棉酚
鼻咽肿瘤
细胞凋亡
L-gossypol
Nasopharyngeal neoplasms
Cell apoptosis
