摘要
目的探讨脑膜瘤恶性程度与活化的信号转导转录活化因子3蛋白质抑制剂（PIAS3）表达的关系。方法72例不同恶性程度脑膜瘤组织标本及13例正常脑组织手术标本，提取总RNA，应用实时荧光定量PCR技术检测PIAS3在不同恶性程度脑膜瘤组织和正常脑组织中的表达，并进行比较。结果PIAS3在正常脑组织中表达很低，在脑膜瘤组织中表达均明显升高，两者之间差异有统计学意义（P〈0．01）。PIAS3在脑膜瘤Ⅰ级、Ⅱ级、Ⅲ级组织中表达水平差异有统计学意义（P〈0．05），但Ⅱ级和Ⅲ级之间差异无统计学意义（P〉0．05）；在侵袭性脑膜瘤组织中PIAS3的表达与浸润程度之间差异无统计学意义（P〉0．05）。结论PIAS3在脑膜瘤组织中高表达，可能与脑膜瘤的发生、发展密切相关。
Objective To explore the relationship between PIAS3 expression and the degree of malignancy of meningiomas. Methods 72 cases of different degree of malignant meningioma and 13 cases of surgical specimens of normal brain tissue were extracted the total RNA, and detected the levels of PIAS3 in different degree of meningioma and normal brain tissue through comparative control study by useing real-time fluorescence quantitative PCR. Results PIAS3 was low expressed in normal brain tissue, however,high expressed in the human brain meningioma with sig- nificant differences（P 〈0. 01 ）. There were also significant differences found in expression level of PIAS3 between meningiomas grade Ⅰ and Ⅱ, or Ⅲ level （P 〈 0. 05 ）, but the differences between meningiomas grade Ⅱ and Ⅲ were not obvious （P 〉 0. 05 ）. And PIAS5 expression was not related with the depth of infiltration in invasive meningiomas （P 〉 0.05 ）. Conclusion PIASS was overexpression in meningiomas, and was be closely related to the occurrence and development of meningiomas.
出处
《中国基层医药》
CAS
2010年第21期2881-2883,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
基金项目：江苏省昆山市社会发展基金（KSl006和KSl009）
