摘要
目的探讨Tribble3(TRIB3)在高糖促进心肌成纤维细胞合成Ⅰ型胶原中的作用。方法体外培养大鼠心肌成纤维细胞,采用实时定量逆转录一多聚酶链反应和酶标记免疫吸附测定法分别测定TRIB3mRNA及Ⅰ型前胶原的mRNA和蛋白的表达,化学合成针对大鼠TRIB3的siRNA,转染大鼠心肌成纤维细胞,观察高糖对心肌成纤维细胞合成Ⅰ型前胶原的变化。结果高糖不仅促进心肌成纤维细胞Ⅰ型胶原mRNA和蛋白质表达,而且增加TRIB3mRNA的表达(P<0.01);TRIB3siRNA抑制TRIB3的表达后,高糖促进Ⅰ型胶原mRNA和蛋白质表达的作用下降(P均<0.01)。结论 TRIB3参与了高糖促进心肌成纤维细胞Ⅰ型胶原合成。
Objective To investigate the role of Tribble 3（ TRIB3） on the production of collagen type Ⅰby cardiac fi-broblasts induced by high glucose. Methods Rat cardiac fibroblasts were cultured in vitro with different concentrations of glucose. mRNA expressions of collagen type Ⅰand TRIB3 were measured by real time-PCR,and protein level of collagen type Ⅰwas determined by enzyme-linked immunosorbent assay. siRNA targeting TRIB3 was transfected into cardiac fibroblasts,and then the production of pro-collagen type Ⅰby cardiac fibroblasts induced by high glucose was detected. Results Both mRNA and protein expressions of collagen type Ⅰwere increased after being induced by high glucose（ P 〈 0. 05） ,and mRNA expression of TRIB3 was also increased（ P 〈 0. 05） . After inhibiting expression of TRIB3 by siRNA,the increased mRNA and protein levels of collagen type Ⅰ induced by high glucose were reversed （ P 〈0.05） . Conclusion TRIB3 is involved in the production of collagen type Ⅰby cardiac fibroblast induced by high glucose.
出处
《山东大学学报（医学版）》
CAS
北大核心
2010年第11期20-23,28,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金资助项目(30871038
81070741
81070192)
山东大学自主创新基金资助项目(2009TS069)
