摘要
用改进的方法从栝楼籽制得一种单链核糖体失活蛋白（ＴＣＫ），研究其性质，ＳＤＳ－ＰＡＧＥ和ＩＥＦ显示为单一条带，分子量为２９ｋＤ，ｐＩ≥９．３，含糖量约为１．７５％。在兔网织红细胞裂解液系统中对蛋白质合成有较强的抑制活性，ＩＣ５０为６．７×１０－１０ｍｏｌ／Ｌ。
A ribosome inactivating protein, named trichokirin(TCK), isolated
and purified to homogeneity from the seeds of Trichosanthes kirilowii by precipitation of
(NH4)2SO4, and CMSepharose FF and Sephadex G-50 column chromatography. TCK is a
glycoprotein containing 1.75% sugar. The molecular weight of TCK is about 29 kD and pI value
is higher than 9.3. Inhibition to protein synthesis was primarily determined by the cell free
system of reticulocyte lysate which display the IC50 was 6.710-10mol/L.
出处
《西北农业学报》
CAS
CSCD
1999年第2期70-72,共3页
Acta Agriculturae Boreali-occidentalis Sinica
基金
农业部属中国农业大学植物生理生化开放实验室资助
关键词
栝楼
核糖体失活蛋白
栝楼素
Trichosanthes kirilowii
Ribosome inactivating protein
Trichokirin
