摘要
依据MabinlinⅡB亚基N端与C端氨基酸序列设计两个简并引物。从马槟榔（CapparismasaikaiLevl．）种子中提取总RNA。经反转录合成cDNA第一链，以此为模板进行多聚酶链式反应（PolymeraseChainReaction，PCR）产物为近22bp的DNA片段。经克隆及序列测定结果表明，该片段为MabinlinⅡB亚基cDNA的185bp。
The first strand of cDNA was synthesized horn total RNA isolated from the seeds of Capparis masikai levl by reverse transcription and was used as a template for polymerase chain reaction with two degenerate primers designed and synthesized according to the amino acid sequences of N-end C-end of B-subunit of Mabinlin Ⅱ. The PCR product was cloned into pBluescript/EcoRV site and sequenced. The result shows the amplified DNA fragment was 185 hp long encoding B-subunit of Mabinlin Ⅱ.
出处
《热带作物学报》
CSCD
1999年第2期57-61,共5页
Chinese Journal of Tropical Crops
基金
国家自然科学基金
关键词
马槟榔
植物甜蛋白
基因克隆与测序
Capparis masaikai Levl sweet-tasting plant protein gene cloning and sequencing
