摘要
目的分析不同结核病患者体内CD4^＋CD25^＋Foxp3^＋调节性T细胞（Tr）表达的变化，探讨其在结核病免疫中的作用。方法对33例结核病患者以及同期30例健康体检者运用流式细胞术检测其外周血CD4^＋CD25“。“和CIM^＋CD25^＋Foxp3^＋Tr表达情况。结果结核组CD4^＋CD25^high和CD4^＋CD25^＋Foxp3^＋Tr检测结果分别为（8．84±2．55）％、（6．30±1．38）％，高于对照组（7．09±1．09）％、（5．22±0．64）％，差别有统计学意义（t=3．57，4．01，P〈0．01）；痰涂阳患者CD4^＋CD25^high和CD4^＋CD25^＋Foxp3^＋Tr检测结果分别为（10．52±3．27）％、（7．18±1．77）％，高于痰涂阴患者（8．21±1．94）％、（5．97±1．06）％，两组问差别均具有统计学意义（t=2．51，2．42，P〈0．05）；初治患者和复治患者间CD4^＋CD25^high和CD4^＋CD25^＋Foxp3^＋检测结果无统计学意义（t=0．03，0．02，P〉0．05）。结论结核病患者体内CD4^＋CD25^high和CD4^＋CD25^＋Foxp3^＋Tr检测结果高于健康人群，提示患者体内存在免疫系统抑制状态。
Objective To investigate the expression of CD4 ^＋ CD25 ^＋ Foxp3 ^＋ regulatory T cell in patients with tuberculosis, and to discover its role in the immune response to mycobacterium tuberculosis. Methods Thirty-three patients with tuberculosis and 30 healthy controls were selected who were consulted in our hospital. Patients were classified by their chemotherapy and smear sputum and CD4 ^＋ CD25 ＋ Foxp3 ^＋ regulatory T cell were detected by flow cytometry. Results Expression of CD4^ ＋ CD25 high and CD4 ^＋ CD25 ＋ Foxp3 ＋ regulatory T cell in experimental group were （ 8.84 ± 2.55 ） % and （ 6.30 ± 1.38 ） % respectively, which were significantly higher than they were in control group （t =3.57,4. 01 ,P 〈0. 01 ）. The expression of CD4 ＋ CD25h^＋gh and CIM ^＋ CD25 ^＋ Foxp3 ^＋ regulatory T cell in patients with positive smear sputum were also significant higher than that in patients with negative smear sputum （t = 2. 51,2.42 ,P 〈 0.05 ）. No significance was founded between the first-visit group and revisit group （ t = 0. 03, 0. 02, P 〉 0.05 ）. Conclusions CD4 ^＋ CD25 hish and CD4 ^＋ CD25 ^＋ Foxp3 ^＋ regulatory T cell in patients with tuberculosis were higher than healthy control, which may result in immune suppression.
出处
《国际免疫学杂志》
CAS
北大核心
2010年第6期472-475,共4页
International Journal of Immunology
基金
福建省卫生厅青年课题项目（2008-2-40）
