摘要
目的探讨hMSH2和MGMT蛋白表达与大肠癌发生、发展的关系及其临床意义。方法采用免疫组化SP法检测169例大肠癌组织(癌组)、36例癌旁黏膜组织(癌旁组)和36例非肠癌患者肠黏膜组织(正常组)hMSH2和MGMT蛋白的表达。结果癌组、癌旁组及正常组hMSH2蛋白的阳性表达率分别为85.21%(144/169)、75.0%(27/36)和22.22%(8/36);MGMT蛋白的阳性表达率分别为71.60%(121/169)、63.89%(23/36)和8.33%(3/36);前两组与第3组蛋白阳性表达率比较差异极显著(P<0.01),且癌组织中hMSH2蛋白与MGMT蛋白的表达呈正相关(r=0.270,P<0.01)。结论在大肠癌发生、发展过程中存在hMSH2和MGMT蛋白异常表达,联合监控这两种蛋白表达可能会有助于大肠癌的诊断。
Objective To investigate the significance of hMSH2 and MGMT proteins in colorectal carcinogenesis. Methods The protein expression of hMSH2 and MGMT in 169 cases of colorectal carcinomas, 36 cases of adjacent mucosa to carcinomas and 36 cases of normal colorectal mucosa was detected by immunohistochemical method and the correlation amang their expression was analyzed by SPSS13.0. Results In carcinoma, adjacent and normal groups, the positive rates of hMSH2 were 85. 21% （144/169）, 75. 0% （27/36） and 22. 22% （8/36）, resoectively, and the rates of MGMT were 71.60% （121/169）, 63.89% （23/36） and 8. 33% （3/36）, respectively. The rates in the former two groups were significantly higher than that in the latter one （ P 〈 0. 01 ） ; the expression of hMSH2 was correlated to the that of MGMT were 71.60% （121/169）, 63.89% （23/36） and 8. 33% （3/36）, respectively. The rates in the former two groups were significantly higher than that in the latter one （ P 〈 0.01 ） ; the expression of hMSH2 was correlated to the that of MGMT in carcinoma group （ r = 0. 270, P = 0. 001 ）. Conclusions There is abnormal expression of hMSH2 and MGMT proteins in eoloreetal carcinomas, indicating that detection of hMSH2 and MGMT proteins may be helpful to diagnose and predict colorectal carcinomas.
出处
《诊断病理学杂志》
CSCD
2010年第5期375-377,共3页
Chinese Journal of Diagnostic Pathology
