摘要
目的: 评价急性社会心理应激对寻常型银屑病患者外周血单核细胞分泌细胞因子的影响。方法:在社会应激测试(TSST)前10 min和测试后1、10、20、60 min采集患者和健康对照者外周血,分离培养外周血单核细胞,用双抗体夹心ELISA法检测培养上清液中IL-6和TNF-α的含量。结果:寻常型银屑病患者和健康对照者TSST测试后20 min外周血单核细胞培养上清液中IL-6、TNF-α的含量均升高,与测试前10 min相比,病例组差异有统计学意义,而对照组无统计学差异。结论: 急性社会心理应激可引起寻常型银屑病患者外周血单核细胞分泌IL-6和TNF-α增加,这可能是社会心理应激使银屑病发生和加重的部分机制。
Objective： To investigate the impact of acute psychosocial stress on the cytokine of patients with psoriasis vulgaris （PsV）. Methods： PsV patients （n = 30） and healthy controls （n = 30） were exposed to a standardized laboratory stressor （trier socialstress test, TSST）. Blood samples were collected 10 rain before and at 1, 10, 20, and 60 min after TSSF. Peripheral blood mononuclear cells （PBMCs） were isolated and cultured. The supemalants were tested for the level of IL - 6 and TNF with sandwich ELISA method. Results： At 20 min after exposure to TSST, the level of IL- 6 and TNF was increased in both PsV patients and controls. Compared with the levels before TSST, the increases were significant in PsV patients, but not in controls. Conclusion： Acute psychosocial stress ean stimulate PBMCs to produce IL- 6 and TNF, which may explain how stress can trigger psoriatic eruption and exacerbation.
出处
《中国麻风皮肤病杂志》
2010年第11期769-771,共3页
China Journal of Leprosy and Skin Diseases
