摘要
用宁夏枸杞－ 宁杞１ 号的髓组织进行离体培养，诱导产生胚性愈伤组织，并用６０Ｃｏγ射线进行诱变，以枸杞根腐菌尖孢镰刀菌（ Ｆｕｓａｒｉｕｍ ｏｘｙｓｐｏｒｕｍ Ｓｃｈｌｅｃｈｔ） 菌株产生的培养滤液为选择剂，筛选抗性变异体，在含毒素６０ ％ 的条件下，一次性获得抗根腐病毒素的愈伤组织，抗性愈伤组织经稳定性分析，其抗性稳定。抗性愈伤组织在含３０ ％ 的毒素培养基中可分化成再生植株，共获得２５ 株，其叶片经用根腐菌分生孢子液接种鉴定，有６ 株抗病；再生植株经过脯氨酸、叶绿素含量分析，二者含量都有所提高；经过过氧化物同工酶酶谱分析，抗性再生植株酶谱带数有增加；
To obtain resistant variants, embryonic calli from pith culture were irradiated with 60 Co γ ray. Resistant calli were screened of cultural filtrate containing 60% toxin of Fusarium oxysporum. Plantlet were then regenerated on differentiation medium containing 30% of toxin. Physiological and biochemical assay showed that both proline and chlorophyll contents increased in resistant variants. Meanwhile, the bands of peroxidase isozyme on PAGE increased as well. Inoculation with suspension of conidium demonstrated that 6 regenerated plantlets were resistant to root rot disease.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第2期163-168,共6页
Acta Phytopathologica Sinica
关键词
枸杞
离体培养
诱变
抗病变异体
再生植株
Lycium barbarum ,in vitro culture,mutagenesis,resistant variants, regenerated plantlets
