摘要
为探讨甲醛、乙醛和丙烯醛对ＤＮＡ损伤机制，应用紫外光谱对甲醛、乙醛、丙烯醛三种醛类化合物引起的ＤＮＡ的加合反应，及与四种单核苷酸作用情况进行了研究。结果表明：甲醛、乙醛均引起小牛胸腺ＤＮＡ的第二个最大吸收峰（２５７ｎｍ）长波方向位移；同时也初步确定了甲醛、乙醛和四种单核苷酸结合的优势反应核苷酸。由于丙烯醛的紫外吸收谱图掩盖了ＤＮＡ的两个最大吸收峰（２１３ｎｍ，２５７ｎｍ），因此不能确切得出丙烯醛与小牛胸腺ＤＮＡ的加合反应情况。
To study the mechanism of DNA damage caused by formaldehyde,acetaldehyde and acrolein,the adduction of calf thymus DNA induced by 3 aldehydes and it's actions on four kinds of deoxyribonucleoside monophosphate (dNMP) were determined by ultraviolet spectrophotometryThe results showed that formaldehyde and acetaldehyde resulted in the displacement of second maximum absorption peak moving to long wavelength for calf thymus DNA,while the dominant dNMP binding with formaldehyde and acetaldehyde was determined alsoBecause two maximum absorption peaks of DNA (213 nm,257 nm) were covered by ultraviolet absorption curve of acrolein,the adductive reaction of DNA with acrolein could not be determined accurately
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1999年第4期189-190,共2页
Journal of Environment and Health
