摘要
利用台架试验模拟汽车行驶工况,收集了发动机使用无铅汽油、含铅汽油燃烧后的尾气颗粒物,对其中吸附的有机物进行了提取,并以Ames试验、中国仓鼠肺细胞(CHL)体外微核实验等方法,从基因、染色体水平检测了两种颗粒提取物的遗传毒性。结果表明,无铅汽油能显著地减少尾气颗粒物的排放,但两种汽油尾气均能诱导鼠伤寒沙门氏菌的回复突变及CHL细胞微核率的升高,呈现一定的剂量—反应关系。比较两种汽油尾气颗粒提取物的毒性,发现单位重量的颗粒物,遗传毒性无显著差别。
Two kinds of gasoline (leaded and unleaded) have been tested on Engine Dynamometer, the total paniculate matter of exhausts were collected, and from which the organic substances were extracted. By using Ames test and micronucleus assay in CHL cells, genotoxicity of two kinds of extracted organic substances were determined from the end points of gene and chromosome, the results showed that unleaded gasoline has the potential of reducing emission of particles, but both kinds of extract could increase the number of histidine-independent colonies in salmonells/ microsomal test and induce micronuclei in CHL cells, and also dose-response relationship were found. As the same particles were being tested, there were no significant differences in genotoxicity between two kinds of extracts.
出处
《上海环境科学》
CAS
CSCD
1999年第7期328-330,327,共4页
Shanghai Environmental Sciences
基金
国家自然科学基金 编号 39670623
关键词
汽车尾气
含铅汽油
无铅泊油
AMES试验
遗传毒性
Vehicle exhaust Leaded gasoline Unleaded gasoline Ames test Micronucleus assay Genotoxicity
