摘要
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source.A high sensitivity cooled charge-coupled device （CCD） camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images.The laser beam performs fast raster scanning using a dual-axis galvanometric scanner.The accuracy of the laser spot position at the source window is within ±200 μm.Based on the phantom experimental results,the spatial resolution is less than 1.7 mm,and the relative quantitation error is about 10%.In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source.A high sensitivity cooled charge-coupled device （CCD） camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images.The laser beam performs fast raster scanning using a dual-axis galvanometric scanner.The accuracy of the laser spot position at the source window is within ±200 μm.Based on the phantom experimental results,the spatial resolution is less than 1.7 mm,and the relative quantitation error is about 10%.In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.
基金
supported by the National High-Tech Research and Development ("863") Program of China under Grant No. 2006AA020801
