摘要
拉曼光谱是物质的特征结构谱,但是在许多情况下,除了激发出拉曼散射光之外,还会激发出强度远大于拉曼散射的荧光,从而限制了拉曼光谱的应用。因此在拉曼检测中对荧光采取抑制措施是十分必要的。在过去的几十年里发展了多种荧光抑制方法,包括荧光淬灭剂法、光漂白法、红外/紫外激发法、偏振调制法、移频激发法、高频调制法、门控法、数值处理法、非线性效应法等。文中概括性地介绍了上述各种技术方法的原理,并扼要地分析比较了各自的性能特点。
Raman spectroscopy provides a fingerprint of the molecules. However, in many cases the fluorescence will also be stimulated apart from the Raman scattering. The fluorescence intensity is often several magnitudes stronger than that of the Raman signal, which severely obstructs the normal application of Raman spectroscopy. Itrs necessary to take some proper fluorescence rejection measures in Raman detection. Many methods and techniques had been proposed and developed to suppress the fluorescence disturbance in the past decades, including fluorescence quenching, photo-bleaching, IR/UV excitation, polarization modulation, shifted excitation Raman spectroscopy, high-frequency modulation, time-gated detection, numerical processing and nonlinear spectroscopic techniques. This paper briefly introduced these techniques and made some analysis on their characteristics and performances.
出处
《光学仪器》
2010年第5期89-94,共6页
Optical Instruments
基金
江苏省高技术研究计划资助项目(BF2009047)
关键词
拉曼光谱
荧光抑制
信噪比
Raman spectroscopy
fluorescence rejection
signal to noise ratio
