摘要
钝顶螺旋藻（Sp.－D）藻胆蛋白的粗提比较方便，用0．001mol／L磷酸盐缓冲液浸提，经反复冻融（三次）处理，可以获得纯度（AλAmax／A280）为0．54的藻胆蛋白粗提液；对粗提液用硫酸铵盐析，可使其纯度略有提高，达到0．59左右，但粗提液中藻蓝蛋白（PC）和别藻蓝蛋白（APC）仍未分离开：进一步通过连续两次羟基磷灰石（HA）柱层析纯化之后，藻蓝蛋白（PC）的纯度可达到4.1，别藻蓝蛋白（APC）的纯度可达到3．5。
The PBP crude extracts of Sp.-D could be easily obtained. Through repeated freezing and thawing (threetimes) in the presence of 0.001mol/L phosphate buffer used as extractant. the purity (Aλmax/A280) of the PBP crudeextract reached 0.54: The purity could reach about 0.59 by salting-out .but PC and APC was not yet seperated fromeach other f Ailer two-time successive hydroxylapatite (HA) column chromatography. the purities of phycocyanin(PC)and allophycocyanin (APC) could reach 4.1 and 3.5 respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1999年第6期48-49,共2页
Food Science
关键词
螺旋藻
提取
钝顶螺旋藻
藻胆蛋白
Spirulina platensis: phycobiliproteins (PBPs) Repeated freezing and thawing Hydroxylapatite(HA)column chromatography
