摘要
目的研讨基层实验室开展细胞遗传学产前诊断技术的可行性。方法普通培养瓶法对350例羊水标本进行细胞培养染色体分析。结果 350例羊水标本1次培养成功340例,1次培养成功率约97.1%。其中引产羊水标本60例,染色体核型分析未见异常;35岁或35岁以上的高龄孕妇羊水标本158例,染色体核型分析结果:1例21-三体;1例47,XXX;1例46,XY,15p+。异常核型约占1.9%。产前筛查唐氏高风险孕妇132例,核型分析结果:2例21-三体;1例46,XY,t(6;19)(q12;q134)。异常核型约占2.3%。结论基层实验室开展细胞遗传学产前诊断技术可行。
Objective Research the feasibility of development cytogenetic prenatal diagnosis technology in theprimary laboratories. Methods The ordinary culture bottle method was carried on the cell culture chromosome analysis for 350 example amniotic fluid specimen. Results Once 340 examples were suecefully raised oncein 350 example amniotic fluid specimen,with a raise success rate of about 97.1 %. these samples include induced labor the amniotic fluid specimen 60 examples, the chromosome karyotype analysis has not seen exceptionally; 158 examples are 35 years-old or above advanced age pregnant woman amniotic fluid specimen , chromosome karyotype analysis result ： 1 example is 2 ltrisomy, 1 example is 47, XXX; 1 example is 46, XY, 15p＋, Abnormal karyotype approximately 1.9 %; 132 examples are Prenatal screening DownSyndrome high risk pregnant woman,karyotype analysis result ： 2 exampte are 21 trisomys, 1 example is 46, XY, t （6 ; 19 ） （ql 2 ; q134） ; Abnormal karyotype approximately 2.3 %. Conclusion The development of cytogenetics prenatal diagnosis experiment in basal unit laboratory is feasible.
出处
《国际检验医学杂志》
CAS
2010年第11期1242-1243,共2页
International Journal of Laboratory Medicine
关键词
细胞遗传学
产前诊断
羊水
细胞培养
染色体
核型分析
eytogenetics
prenatal diagnosis
amniotic fluid
cell culture
chromosomes
karyotyping
