摘要
目的主要探讨ｒａｓｐ２１表达和ＤＮＡ含量测定的预后意义。方法采用流式细胞术对４１例进展期胃癌的石蜡包埋标本进行了ｒａｓｐ２１表达和ＤＮＡ含量的定量检测。结果ＤＮＡ二倍体组的５年生存率（３５．７％）较异倍体组高（２２．２％，Ｐ＜０．０５），ｒａｓｐ２１低表达组（Ｆｉ≤Ｘ）的５年生存率（２９．１％）较ｒａｓｐ２１高表达组（Ｆｉ＞Ｘ）的５年生存率（１４．３％）高（Ｐ＜０．０５）；高ｒａｓｐ２１表达异倍体胃癌（５年生存率１４．４％）较低ｒａｓｐ２１表达异倍体胃癌（５年生存率２８．６％）预后不良（Ｐ＜０．０５）。结论ＤＮＡ倍体类型和ｒａｓｐ２１表达，尤其是双参数同步分析，可望成为评估胃癌预后的客观指标之一。
Objective To evaluate the prognostic significance of nuclear DNA content and ras p21 expression in gastric cancer.Methods Flow Cytometry was used to detect DNA content and ras p21 expression in paraffin-embeded section of tumors obtained surgically from 41 advanced gastric carcinomas.Results A better 5-year survival rate was observed in DNA diploid ( 35.7% ) than in the DNA aneuploid group ( 22.2% ,p< 0.05 ).Patients with the lower ras p21 expression (Fi≤) survived significantly longer (5-year survival rate, 29.1% ) than those with higher ras p21 expression (FI>, 14.3% ,P< 0.05 ).Also,among the DNA aneuploid tumors,the lower ras p21 expression (Fi≤) survived significantly longer (5-year survival rate of 28.6% ) than those with higher ras p21 expression (Fi>,5-year survival rate of 14.4% ).Conclusions The results suggest that DNA ploidy patterns and the ras p21 expression,especially analyzed simultaneously,may be one of the important objective data for the assessment of the prognosis of the gastric carcinoma.
出处
《中国肿瘤临床与康复》
1999年第3期6-8,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
胃癌
癌基因产物
DNA倍体
预后
流式细胞术
Gastric carcinoma ras oncogene products p21 DNA ploidy Prognosis Flow Cytometry
