摘要
目的初步探讨梓醇对慢性脑缺血所致白质损伤的保护作用及机制。方法成年雄性Wistar大鼠24只,分为4组:假手术组,缺血组,生理盐水组和梓醇组。通过结扎大鼠双侧颈总动脉建立慢性脑缺血模型,同时腹腔注射5mg/kg梓醇或生理盐水予以治疗,每天1次,连续10d。术后30d处死,然后利用卢卡斯快蓝(luxol fast blue,LFB)染色法和免疫组织化学技术分别观察梓醇对脑白质髓鞘以及髓鞘碱性蛋白(myelin basic protein,MBP)、腺瘤性息肉蛋白(adenoma-tus polyposis coli,APC(CC-1)和星形胶质纤维酸性蛋白(glial fibrillary acidity protein,GFAP)表达的影响。结果与假手术组比较,在缺血组和生理盐水组,LFB染色变浅[(37259.6±6626.6)、(36554.4±7865.7)vs(90470.5±5004.6)],MBP表达下降[(24016.5±3794.8)、(23923.6±4605.1)vs(65055.4±6839.9)],CC-1阳性的少突胶质细胞(oligo-dendrocytes,OLs)减少[(33.0±9.6)、(32.0±9.8)vs(100.0±11.2)],GFAP阳性的星形胶质细胞增多[(52.0±7.4)、(50.0±9.4)vs(14.0±6.6)],均有显著差异(P<0.01);梓醇治疗后能显著地减轻白质损伤,它能使LFB染色加深(86687.6±6619.1),MBP表达升高(61604.5±6319.9),CC-1阳性细胞数增加(91.0±8.4),GFAP阳性细胞数减少(17.0±7.8),与生理盐水组比较均有显著差异(P<0.01)。结论梓醇可通过抑制星形胶质细胞的增生进而抑制炎症反应来保护慢性脑缺血所致的白质损伤。
Objective To study the protective effect of catalpol on white matter injury due to chronic cerebral ischemia in rats and its mechanism.Methods Twenty-four adult male Wistar rats were randomly divided into sham-operation group,ischemia group,vehicle group and catalpol group,6 rats in each group.A chronic cerebral ischemia model was established by ligating bilateral common carotid arteries.Ischemic rats were treated by intraperitoneal injection with catalpol or vehicle（normal saline）at 5 mg/kg per day for 10 days and killed 30 d after operation.Luxol fast blue（LFB）staining and immunohistochemical staining were used to observe the effects of catalpol on expressions of myelin sheath,myelin basic protein（MBP）,adenomatus polyposis coli [APC（CC-1）],and glial fibrillary acidity protein（GFAP）in white matter of rats.Results The LFB staining was weaker,the expression level of MBP and the number of CC-1-positive oligodendrocytes（OLs）were lower,and the number of GFAP-positive astrocytes was higher both in ischemia group and in vehicle group（37 259.6±6 626.6 vs 36 554.4±7 865.7,24 016.5±3 794.8 vs 23 923.6±4 605.1,33.0±9.6 vs 32.0±9.8,52.0±7.4 vs 50.0±9.4）than in sham-operation group（P0.01）.The damage to white matter was more significantly alleviated in catalpol group than in vehicle group（P0.01）.The LFB staining was stronger（86 687.6±6 619.1）,the expression level of MBP（61 604.5±6 319.9）and the number of positice CC-1 cells（91.±8.4）were higher,and the number of GFAP positive cells was lower（17.0±7.8）in catalpol group than in vehicle group（P0.01）.Conclusion Catalpol protects against white matter injury due to chronic cerebral ischemia in rats by inhibiting the proliferation of astrocytes and inflammatory reactions.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第21期2273-2276,共4页
Journal of Third Military Medical University
基金
国家自然科学基金(31071056)~~
关键词
梓醇
脑缺血
髓鞘
白质
大鼠
catalpol
cerebral ischemia
myelin sheath
white matter
rat
