摘要
为阐明秦公散的抗炎作用机制,本试验采用流式细胞术检测了LPS刺激的内皮细胞黏附分子E-选择素和ICAM-1的表达,用一氧化氮(NO)测定试剂盒检测LPS刺激的内皮细胞培养上清液中NO的含量。结果表明,LPS刺激的内皮细胞可显著提高E-选择素和ICAM-1的表达(P<0.01),显著提高培养上清液中NO的含量(P<0.05);用秦公散及其含药血清处理后则明显抑制LPS诱导的E-选择素、ICAM-1的表达和NO分泌(P<0.05)。表明秦公散可通过抑制黏附分子的表达及NO分泌量发挥抗炎作用。
To state the Anti-inflammatory effects mechanism of Qingongsan,the flow cytometry was adopted to detect the expression of E-selectin and ICAM-1 in the endothelial cell activated by LPS and the NO determining kit was adopted to determine the concentration of NO in cultivation supernatant.Results： the human umbilical vein endothelial cells were cultured with LPS together can remarkably increase the expression of E-selectin and ICAM-1（P0.01）,treated by crude extract of Qingongsan and the serum containing drugs can inhibit the expression of E-selectin,ICAM-1 and increase secretary of NO induced by LPS（P0.05）.Qingongsan can educe its anti-inflammation function though inhabiting the expression of adhesion molecule and excessive secretion of NO.
出处
《动物医学进展》
CSCD
北大核心
2010年第11期67-70,共4页
Progress In Veterinary Medicine
基金
呼和浩特市科技局项目(2005-农-7)
