摘要
目的 观察新生鼠缺氧缺血性脑损伤（ＨＩＢＤ） 后脑组织中神经元特异性烯醇化酶（ＮＳＥ） 、胶质纤维酸性蛋白（ＧＦＡＰ） 、Ｓ１００ 蛋白（Ｓ１００）ｍ ＲＮＡ表达的变化。 方法 采用７ 日龄ＳＤ大鼠缺氧缺血性脑损伤模型，用逆转录聚合酶链反应（ＲＴＰＣＲ） 方法，动态观察脑组织中ＮＳＥ、ＧＦＡＰ、Ｓ１００ 蛋白ｍＲＮＡ 水平变化。 结果 ＮＳＥ ｍ ＲＮＡ 转录高峰出现在缺氧缺血后２４ 小时；ＧＦＡＰ、Ｓ１００ 蛋白ｍ ＲＮＡ转录高峰出现在缺氧缺血后４８ 小时，以后都随时间变化逐渐下降。 结论 在缺氧缺血性脑损伤后有ＮＳＥ、ＧＦＡＰ、Ｓ１００ 蛋白ｍ ＲＮＡ 表达增加。提示ＨＩＢＤ 时在血和脑脊液中ＮＳＥ、ＧＦＡＰ、Ｓ１００ 水平的升高，除从损伤细胞漏出外。
Objective To observe the expression of mRNA for neuron specific enolase (NSE), glial fibrillary acidic protein(GFAP)and S 100 protein (S 100) in cerebral tissue after hypoxic ischemic brain damage(HIBD) in newborn rat. Methods The dynamic changes of expression of mRNA for NSE、GFAP and S 100 protein in the cerebral tissue were studied by using 7d old SD rat hypoxic ischemic brain damage model and RT PCR technique. Results The peak expression of mRNA for NES and for GFAP and S 100 protein occurred 24 and 48 hours after HIBD respectively, and their expression decreased gradually. Conclusion The expression of mRNA for NSE、GFAP and S 100 protein increased significantly after HIBD. It suggests that the increased level of NSE、GFAP and S 100 protein in serum and cerebrospinal fluid (C.S.F) maybe reflect the increase of transcription and transnational except for the leakage of damaged cell.
出处
《中华围产医学杂志》
CAS
1999年第2期89-92,共4页
Chinese Journal of Perinatal Medicine
基金
国家"九五"攻关课题
关键词
S-100蛋白
缺氧缺血性脑病
新生儿
NSE
GFAP
Neuron specific enolase Glial fibrillary acidic protein S 100 protein Hypoxic ischemic brain damage
