摘要
采用改进的Ｆｅｅｎｅｙ氏落体打击致Ｗｉｓｔａｒ大鼠脑挫伤模型，利用免疫组织化学染色，观察大鼠实验性脑挫伤后ＧＦＡＰ、ＰＣＮＡ的改变，用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量，ＳＡＳ统计软件分析，得出脑挫伤后ＧＦＡＰ、ＰＣＮＡ变化的时间规律性，为脑挫伤形成时间推断提供新的手段。ＧＦＡＰ阳性细胞面积和灰度伤后呈上升趋势，伤后３ｈ，即有显著增加（Ｐ＜０．０１），至４天时，阳性细胞灰度，面积达到峰值（Ｐ＜０．０１），伤后７天仍保持较高水平（Ｐ＜０．０１）。ＰＣＮＡ阳性细胞伤后１２ｈ出现，灰度呈上升趋势，面积呈下降趋势，伤后３天时达到最大值（Ｐ＜０．０１）。因此，ＧＦＡＰ、ＰＣＮＡ免疫组织化学染色阳性细胞的数量，灰度，面积改变有时间性规律；推断２～７天的脑挫伤则以ＧＦＡＰ、ＰＣＮＡ改变为主；脑挫伤后星形胶质细胞数目增多，只有很少一部分为星形胶质细胞增殖而来。
After the model of
experimental brain contusion was set up,the changes of GFAP,PCNA were studied by
immunohistochemistry SP method combined with image quantity analysis.After contusion,the
gray degrees and areas of GFAP-positive cells increased significantly at the 3rd hour,reached
the highest level in the 4th day and kept until the 7th day.PCNA-positive cells appeared in the
12th hour, simultaneously, their gray degrees tended to increase and positive areas tended to
decrease. It showed regular changes of GFAP,PCNA with various survival times, and these
changes were useful in the diagnosis of brain contusion especially in the period from the
second to 7th day after injury.The numbers of astrocytes increased and reached the highest
level in the 3rd day after contusion while the reactive proliferation of astrocytes is only a minor
phenomenon.
出处
《法医学杂志》
CAS
CSCD
1999年第2期65-66,共2页
Journal of Forensic Medicine
基金
辽宁省科学技术基金
关键词
脑挫伤
免疫组化
图像分析
brain contusion,immunohistochemistry,Glial fibrillary acidic protein
(GFAP),proliferating cell nuclear antigen (PCNA),image analysis
