摘要
目的 研究聚乙二醇IFN-α2a（Peg-IFN-α2a）治疗后完全应答乙型肝炎患者外周血中Th1与Th2细胞频数变化.方法 抽取应用Peg-IFN-α2a治疗发生完全应答乙型肝炎患者7例、健康对照者9名、未进行治疗的乙型肝炎患者9例的静脉血,分离血清及外周血单核细胞（PMBC）,检测血清中HBV标志物、HBV DNA、ALT以及外周血中的CD3＋CD4＋IFN-γ＋T细胞、CD3＋CD4＋IL-4＋T细胞的频数.结果 7例患者发生HBsAg转换的时间最短为36周,最长为49周,平均为（39.28±6.44）周.患者外周血中CD3＋CD4＋IFN-γ＋T细胞的频数表现为持续下降,但6个月后仍高于健康对照;而CD3＋CD4＋IL-4＋T细胞的频数在发生应答的时间点升高,明显低于健康对照,6个月后下降.结论 应用Peg-IFN-α2a治疗后发生完全应答的患者,体内Th1类免疫应答下降而Th2类应答不足,体内仍存在一定程度的炎症反应.
Objective To investigate the feature of Th1 and Th2 cells in chronic hepatitis B（CHB） patients complete responsed after pegylated interferon α-2a treatment. Methods Serum and PBMC were obtained from 7 enrolled CHB patients, 9 health controls and 9 untreated hepatitis B patients, and monitored for ALT, HBV DNA, HBV markers as well as the frequency of CD3＋ CD4＋ IFN-γ＋ T cells, CD3＋ CD4＋ IL-4＋ T cells. Results All the 7 patients occured HBsAg conversion, the short est conversion time was 36 weeks, and the long est conversion time was 49 weeks, the average time was（39.28 ± 6.44）weeks. The frequency of CD3＋CD4＋IFN-γ＋T cells in 7 completely responsed patients decreased gradually, but it was still higher than the health control group in followed 6 months; the frequency of CD3＋CD4＋IL-4＋T cells increased in whole response time point but still lower than the health control group and decreased in followed 6 month. Conclusions In patients with whole response to pegylated interferon α-2a, Th1 immune reaction decreases and Th2 immune reaction is insufficient which correlates with the hepatic inflammation.
出处
《国际流行病学传染病学杂志》
CAS
2010年第5期300-302,共3页
International Journal of Epidemiology and Infectious Disease
