摘要
目的观察大黄虫对药不同剂量含药血清对原代培养人子宫肌瘤细胞增殖的影响。方法原代培养并建立人子宫平滑肌瘤细胞系,采用中药血清药理学方法处理细胞,以MTT比色法检测含药血清作用于子宫肌瘤细胞12,24,48,72h的光密度值,计算细胞存活率。结果大黄虫对药含药血清可直接抑制子宫肌瘤细胞增殖。结论大黄虫对药含药血清可直接抑制子宫肌瘤细胞增殖,子宫肌瘤细胞是大黄虫对药含药血清发挥治疗作用的靶细胞。
Objective To observe the effects of different dosages of medicated sera of Dahuang and Zhechong on the proliferation of human leiomyoma cells in vitro.Methods Human leiomyoma cells were cultured in vitro.Treated the cells with Chinese herbs serum pharmacology method.The MTT assay was adopted to investigate the survival rate of 12 h,24 h,48 h,72 h after the cells were treated.Results Serum containing Dahuang and Zhechong showed an direct inhibitory action on proliferation of uterine leiomyoma cells.Conclusion Dahuang and Zhechong medicated serum has inhibitory effect on proliferation of hysteromyoma cells,which indicates hysteromyoma cells are target cells.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第11期2872-2873,共2页
Lishizhen Medicine and Materia Medica Research
基金
陕西省教育厅基金(No.05JK184)
陕西省中医药管理局基金(No.2007029)
关键词
大黄
虫
子宫肌瘤
细胞培养
含药血清
Dahuang
Zhechong
Leiomyoma
Cell culture
Medicated serum
