摘要
目的采用正交设计与星点设计-效应面法两种实验设计方法,优选淫羊藿提取工艺,并对设计方法进行比较。方法以淫羊藿苷提取量为因变量,乙醇浓度、回流时间和溶剂(倍)量为自变量,优选提取工艺。结果正交实验设计确定最佳工艺是12倍量60%乙醇回流提取两次,3 h/次;星点设计效应面优化法确定最佳工艺是12倍量50%乙醇回流提取两次,160 min/次。结论星点设计-效应面法在该提取工艺研究中优于正交实验设计法,为其应用于中药提取工艺优化的可行性提供了依据。
Objective To optimize the process of extacting effective constituents from Epimedii Herba by orthogonal experimental design and central composite design-response surface method.Methods Independent variables were concentration of ethanol,extraction time,times of reflux and solvent fold.Dependent variable was extraction rate of icariin in Epimedii Herba.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables.Results The result of the orthogonal experimental design was 60% ethanol,180 minutes for reflux,12 fold of solvent and 2 times for extraction.The other was 50% ethanol,160 minutes for reflux,12 fold of solvent and 2 times for extration.Conclusion The Central Composite Design-Response Surface Method is highly predictive in experimental design.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第11期2766-2768,共3页
Lishizhen Medicine and Materia Medica Research
基金
天津市自然科学基金(No.09TCYBTC13500)
天津市高等学校科技发展基金计划项目(No.20060302)
天津卫生局资助项目(No.07005)
关键词
正交设计
星点设计-效应面法
淫羊藿苷
提取工艺
Orthogonal experimental design
Central Composite Design-Response Surface Method
Icariin
Extraction process
