摘要
目的研究环维黄杨星D(CVB-D)透皮贴剂对实验性新西兰兔心肌缺血模型的影响。方法通过结扎冠状动脉左旋支制备心肌缺血动物模型,将15只新西兰兔随机分为3组,以模型组为对照,分别考察CVB-D贴剂组和CVB-D口服给药组,对试验动物心电图、血浆乳酸脱氢酶(LDH)、肌酸激酶(CK)活性、丙二醛(MDA)含量的影响,并对缺血部位心肌壁内膜进行病理学检查。结果模型组动物心电图T波抬高,ST段偏移明显,血浆LDH、CK活性和MDA含量明显升高(P<0.01),坏死心肌细胞表现为核固缩与核溶解,细胞浆崩解为细颗粒状。贴剂给药组和口服给药组均能显著抑制血浆LDH、CK活性和MDA含量升高(P<0.05),贴剂给药组在32 h内药效作用较为平稳,但与口服组比较并无统计学差异(P>0.05)。结论 CVB-D透皮贴剂对新西兰兔心肌缺血性损伤具有保护作用。
Objective To explore the pharmacological actions of Cyclovirobuxine D（CVB-D） transdermal patch in rabbit models with myocardial ischemia.Methods The animal model with myocardial ischemia was prepared by ligating the left branch of cornary artery of L-branch.15 New Zealand albino rabbits were randomly divided into 3 groups： CVB-D patch group,oral CVB-D group and model group.Electrocardiogram,activities of plasma lactic dehydrogenase（LDH）,creatine kinase（CK） and the malondialdehyde content（MDA） in experimental animals were investigated.The endomyocardial injury to myocardial ischemia was performed by pathological examination.Results The results of the model group were summarized as follow： ECG T-wave elevation,significant ST segment deviation,the significant plasma LDH,CK activities and the MDA content（P0.01）,karyopyknosis and karyolysis in myocardial necrosis cells,finely granular disintegration of cytoplasm.Patch group and oral group could inhibit the inerease of plasma LDH,CK activities and MDA content（P0.05）.The pharmacodynamic effects of patch group were relatively stable within 20 hours,but there had no statistical significant difference between patch group and oral group（P0.05）.Conclusion CVB-D patch has protective effect against myocardial ischemia in New Zealand albino rabbit model.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第11期2723-2725,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30672669
30371783)
广州市科技攻关计划(No.200623-E5021)
关键词
环维黄杨星D
透皮贴剂
心肌缺血
药效学
Cyclovirobuxine D
Transdermal patches
Myocardial ischemic
Pharmacodynatics
