摘要
目的从人胎盘组织中分离培养获得间充质干细胞(MSCs),并进一步研究其对异体T淋巴细胞细胞因子白细胞介素2(IL-2)、γ-干扰素(IFN-γ)分泌的影响。方法首先从胎盘组织中分离培养胎盘间充质干细胞(PM-SCs),在体外观测其形态,并通过细胞表面抗原表达、分化潜能等特征进行鉴定;然后将体外分离培养扩增的PMSCs按照不同比例加入双向混合淋巴细胞培养体系(MLR)中,共同培养3d后,用ELISA法检测各组MLR上清中细胞因子IL-2与IFN-γ的含量。结果从人胎盘组织中分离培养获得MSCs,具有与骨髓间充质干细胞(BMSCs)极为相似的细胞形态及细胞表面标志,并且还具有与BMSCs相似的跨胚层分化能力,能在一定条件下被诱导分化出神经元样细胞。PMSCs与同种异体混合淋巴细胞共同培养后,能有效抑制MLR中T淋巴细胞细胞因子IL-2与IFN-γ的分泌(P<0.05)。结论胎盘组织是MSCs的有效来源,PMSCs具有与BMSCs相似的生物学特性及免疫调节机制,为MSCs的研究提供了又一重要的细胞来源。
Objective To isolated and cultivated mesenchymal stem cells from human placenta tissue,and then investigate of the effect of MSCs from human placenta tissue on allogeneic T lymphocytes cytokines secretion. Methods Firstly human placenta-derived mesenchymal stem cells（ PMSCs） from placenta tissue were isolated,their morphology and identified cell surface antigen expression were observed by FACS analysis and their differentiation potency was observed in vitro. The PMSCs were cultivated and expanded in vitro,then used in two-ways mixed lymphocyte reation（ MLR） essay,according to different ratio between PMSCs and lymphocytes. The secretion of IL-2 and IFN-γ in each group was evaluated by ELISA after 3 days’coculture. Result MSCs isolated from placenta tissue had the similar morphology and cell surface markers with bone marrow mesenchymal stem cells（ BMSCs） ,and were able to be induced into neuron-like cells. In MLR essay,PMSCs were able to inhibit IL-2 and IFN-γ secretion in vitro（ P 0. 05） . Conclusion The human placenta tissue is proved to be a useful source of the MSCs. PMSCs had the similar biological characteristics and immuno-regulatory effect with BMSC,which will provide alternative cell source for the mesenchymal stem cells.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2010年第5期943-946,963,1125,共6页
Suzhou University Journal of Medical Science
