摘要
目的:调查2种抗菌制剂外排泵基因(mdfA、qacE△1)和7种可移动遗传元件(traA、trbC、IS1133、ISEcp1、tnpU、tnp513、qacE△1)在多耐药肺炎克雷伯菌中的存在和变异情况。方法:收集绍兴市人民医院2007年10月-2009年6月住院患者标本中分离的多耐药肺炎克雷伯菌共20株,采用聚合酶链反应(PCR)及序列分析的方法分析上述8种基因。结果:20株多耐药肺炎克雷伯菌共检出7种基因,mdfA、traA、trbC、ISEcp1、tnpU、tnp513、qacE△1基因的阳性株数分别为18株(90.0%)、2株(10.0)、16株(80.0%)、14株(70.0%)、8株(40.0%)、11株(55.0%)、18株(90.0%),只有IS1133基因未能检出。且这7种阳性基因以7种阳性模式存在,其中(mdfA+trbC+ISEcp1+qacE△1)模式的检出率最高,共6株(30.0%)。结论:抗菌制剂外排泵基因和可移动遗传元件阳性率很高,这是导致本组菌株呈多耐药的一个重要原因。
Objective：To investigate the distribution and variety of 2 kinds of drug efflux genes（mdfA,qacE△1）and 7 kinds of mobile genetic elements（traA,trbC,IS1133,ISEcp1,tnpU,tnp513,qacE△1） in multidrug-resistant Klebsiella pneumoniae strains.Methods： From October 2007 to June 2009,20 strains of multidrug-resistant Klebsiella pneumoniae were collected from Shaoxing People′s Hospital in China.Eight kinds of genes mentioned above were analyzed by PCR and verificated by DNA sequencing.Results： Seven kinds of positive genes were detected in 20 strains of Klebsiella pneumoniae.And 18（90.0%）,2（10.0%）,16（80.0%）,14（70.0%）,8（40.0%）,11（55.0%）,18（90.0%） strains were detected to carry mdfA,traA,trbC,ISEcp1,tnpU,tnp513,qacE△1,respectively.However,IS1133 couldn′t be detected.Furthermore,7 kinds of positive modes were classified according to the presence of 7 kinds of positive genes,and the mode（mdfA＋trbC＋ISEcp1＋qacE△1） had the highest rate： 6 strains（30.0%）.Conclusion： The fact that the positive rate of drug efflux genes and mobile genetic elements was high in Klebsiella pneumoniae strains was an important factor to induce multidrug-resistant strains.
出处
《中国卫生检验杂志》
CAS
2010年第11期2811-2814,共4页
Chinese Journal of Health Laboratory Technology
关键词
肺炎克雷伯菌
外排泵基因
可移动遗传元件
多耐药
亚型
Klebsiella pneumoniae
Drug efflux gene
Mobile genetic element
Multidrug-resistance
Subtype
