摘要
目的用细胞毒实验对幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，ＨＰ）致病机制作进一步研究。方法６２株临床分离的幽门螺杆菌，４３株的肉汤培养滤液（０．２２ｕｍ微孔滤膜）作用于体外培养的Ｖｅｒｏ细胞，能使其产生胞浆空泡样变。随着作用时间延长，空泡数目逐渐增多，体积逐渐增大，４天后细胞逐渐死亡。据此将ＨＰ分为有空泡毒作用的ＨＰ（Ｔｏｘｉｎ＋）及没有空泡毒作用的ＨＰ（Ｔｏｘｉｎ－）。二类ＨＰ肉汤培养滤液的尿素酶活性无明显差异（Ｐ＞０．０５）。结果ＨＰ的这种空泡毒作用不同于已知的ＨＰ的致病因素，而是一种新近发现的致病因素。ＨＰ相关消化性溃疡、十二指肠球部溃疡、胃炎患者，ＨＰ（Ｔｏｘｉｎ＋）分别占其ＨＰ感染总数７８．２６％、８４．３８％、４２．８６％。
OBJECTIVE Cytotoxic test was used to further study the pathogeny of Helicobacter pylori (HP). METHODS HP strains were obtained by culture from patients Vero cell intracytoplasmic vacuolization was induced by Broth Culture Filtrate (BCF, 0.22um pore filter) of 43 of 62 HP strains obtained from patients. The number of vacuole increased and the size grew larger with the vacuolated toxin. The vacuolated Vero cells died after 4 days. On the basis of vacuolated toxicity, HP strains were divided into two groups, HP (Toxin +) and HP (Toxin -). RESULTS There was no significant difference (P>0.05) of urease activity in the BCF between HP (Toxin +) and HP (Toxin -). The vacuolated effect of HP (Toxin +), which was a pathogeny found recently, was different from the effects of HP measured ever. The infective rate of HP (Toxin +) among the patients with HP-associated-peptic ulcer, duodenal ulcer, gastritis were 78.26%, 84.38% and 42.86% separately. CONCLUSION HP (Toxin +) infection is associated closely with peptic ulcer disease.
出处
《中华医院感染学杂志》
CAS
CSCD
1999年第2期80-82,共3页
Chinese Journal of Nosocomiology
