摘要
采用逆转录聚合酶链反应（PCR）技术自SD大鼠肝细胞中克隆出全长1125个bP的编码主要组织相容性抗原Ⅰ类复合体分子（MHC－Ⅰ）cDNA。经限制性内切酶图谱分析证实后，定向插人表达载体pBV220，并筛选出带有插人片断的阳性克隆。为研究在原核或真核表达系统中的表达，及其在抗原识别，免疫应答中的作用奠定了基础。
The total RNA was extincted from the rat liver cells. Accolading to the puLblished seqUence of rat MHC- I(rMHC- I ), a couple of primers for rMHC- 1 was designed and synthesized. POlylnerase chain reaction for reversetranSCription obtained a specific band of rMHC- 1 cDNA fragment from rat liver cells. The obtained fragment was inserted to PBV220 plasthed and tranSformed to E. colt DH5a. Restrichon e^e digestion and PCR identified it as rat rMHC1 with the whole ~ng flame of 1 125 base pairs.
出处
《汕头大学医学院学报》
1999年第1期7-9,共3页
Journal of Shantou University Medical College
