摘要
从传统资源研究方法和"meta-技术"两方面阐述了微生物勘探的必要性,介绍微生物勘探的主要内容与研究方法,讨论微生物勘探对微生物资源研究的意义与价值及其发展趋势。
The development of molecular biology and bioinformatics have greatly changed the concept,strategy and technology of microbial resource investigation.Up to present,bioprospecting approaches based on PCR,DNA hybridization,and other technologies,as well as their combinational methods have expanded the extent and depth of microbial resources,which the traditional investigation of culture resources changed to the microbial investigation including uncultured microbes,and their genes and metabolites.This brief review focuses on the necessity of bioprospection from the points of traditional microbial techniques and ＇metaapproach＇,introduces the concept and techniques of bioprospecting on microbial resources,and the significance and future perspectives.
出处
《热带作物学报》
CSCD
2010年第8期1420-1426,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.30560005)
"863"计划(No.2007AA09Z415)
教育部新世纪优秀人才项目(No.NCET-05-0756)
中央级公益性科研院所基本科研业务费专项资金项目(No.ITBBZD0753)资助
关键词
生物勘探
未培养微生物
微生物资源
Bioprospecting
Uncultured microorganisms
Microbial resources
