摘要
以荸荠试管苗为材料,在培养基中添加不同浓度的亚硒酸钠溶液,研究硒对荸荠组培苗的生长及SOD和POD活性的影响。用于培养幼苗的培养基为MS+6-BA(1.0mg/L)+NAA(0.1mg/L)+6%琼脂+30%蔗糖,在培养基中添加不同浓度的亚硒酸钠溶液后,测定荸荠幼苗的长势及SOD和POD等酶活性及MDA含量。结果显示,硒浓度为45mg/L时,荸荠组培苗的生长势较好,SOD和POD等酶的活性相对较高,MDA含量最低;硒浓度低于45mg/L时,对SOD和POD起激活作用,可促进生长;硒浓度高于45mg/L时,对SOD和POD活性及组培苗的生长有抑制作用。表明只有适当的硒浓度,才能增强SOD和POD活性,促进生长。
This article primarily studied effects of Se on growth and activity of SOD and POD in Water chestnut;The medium for tissue culture of Water chestnut is MS ＋ 6-BA （1.0 mg/L） ＋ NAA （0.1 mg/L） ＋ 6 g/L agar＋30 g/L sucrose.After adding different concentrations of Se in the medium,the change of SOD and POD activities and the contents of MAD and the growth vigor were determined;When the selenium content was 45 mg/L,SOD and POD activities were higher,the growth vigor was better and the MAD content was decreased;Only appropriate selenium content could enhance activity of SOD and POD and promote growth.
出处
《热带作物学报》
CSCD
2010年第8期1368-1371,共4页
Chinese Journal of Tropical Crops
基金
湖北省重点实验室开放课题"生物资源保护与利用"(No.2007019)资助
关键词
硒
荸荠
试管苗
生长
SOD活性
POD活性
Selenium
Water chestnut
Test-tube plantlet
Growth
SOD activities
POD activities
