摘要
目的:探讨国医大师经验方"抗癌扶正方"的抗肝癌作用及机制。方法:提取抗癌扶正方,针对人肝癌细胞SMMC-7721,经过细胞培养、MTT法、流式细胞仪检该方对肝癌细胞生长抑制率的影响及对突变型P53基因表达的调控作用。结果:抗癌扶正方具有良好的抑制肝癌细胞生长作用,尤其以1mg/mL,10mg/mL剂量组的效果最佳,作用程度与化疗药物类似。此方可以下调凋亡抑制基因突变型P53的表达。结论:抗癌扶正方能够抑制人肝癌细胞SMMC-7721活性。其机理在于降低肝癌凋亡抑制基因的表达,促进细胞凋亡的产生。
Objective：To Probe the anticancer effect of Kangai Fuzheng Prescription which is from national experienced expert＂ Zhou Zhongying＂. Methods：Applying Kangai Fuzheng Prescription and human hepatoma SMMZ-7721 cell to cell experiment,detecting the growth inhibiting rate. Detecting the expression of P53 by flowcytometry. Results：It is shown that Kangai Fuzheng Prescription has function to inhibit human hepatoma SMMZ-7721 cell and to decrease the expression of gene inhibitor P53. Conclusion：Kangai Fuzheng Prescription could inhibit the growth rate of human hepatoma SMMZ-7721. The mechanism maybe decrease expression of apoptosis gene,promote apoptosis.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第11期2215-2217,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
江苏省自然科学基金项目(BK2007240)
国家科技支撑计划(2006BAI11B08-01)
