摘要
为了评价铝胶、蜂胶及白油佐剂对副猪嗜血杆菌的免疫增强效果,我们将铝胶、蜂胶和白油3种佐剂分别与副猪嗜血杆菌血清5型菌株联合制备灭活疫苗,然后接种雄性成年家兔,采用ELSIA方法分别检测3种灭活苗在免疫期间副猪嗜血杆菌P5蛋白的抗体效价。结果显示:3次免疫后,白油佐剂免疫组的平均效价为1∶4000,铝胶佐剂免疫组的平均效价为1∶700,蜂胶佐剂免疫组的平均效价为1∶100,无佐剂对照组的平均效价为1∶100。说明这3种佐剂中免疫增强效果最好的为白油,铝胶次之。
To evaluate the immunoenhancement of aluminium hydroxide,propolis and oil-emulsion on Haemophilus parasuis,we combined haemophilus parasuis（serotypes 5） and aluminium hydroxide,propoils and oil-emulsion to prepare inactivated vaccine,and inoculated male rabbits.ELISA method was used to detect the antibody titer of P5 protein of haemophilus parasuis in the duration of immunity.After third immunity,the average antibody titer of oil-emulsion group,aluminiun hydroxide,propolis and control group were 1∶4 000,1∶700,1∶100,1∶100 respectively.The conclusion showed that the effect of oil-emulsion was best,the aluminium hydroxide came second.
出处
《四川畜牧兽医》
2010年第11期26-28,共3页
Sichuan Animal & Veterinary Sciences
基金
"十一五"国家科技支撑计划重大项目(2006BAD06A11)
关键词
副猪嗜血杆菌
灭活疫苗
佐剂
Haemophilus parasuis
Inactivated vaccine
Adjuvant
