摘要
采用新近发展的ｃＤＮＡ代表性差异分析法筛选鼻咽癌中不表达的或表达降低的ｃＤＮＡ序列。结果显示：有９个与已知基因高度同源的ｃＤＮＡ序列。通过对这些已知基因的结构和功能分析，发现有与细胞骨架成分相关的基因：αａｃｔｉｎｉｎ，ｅｚｒｉｎ和细胞角蛋白１３；直接与瘤基因和抑瘤基因相互作用的基因：鲨烯合成酶和ＴＲＩＰ１基因；直接参与ＤＮＡ合成以及调控基因转录和翻译的基因：ＴＡＦⅡ６８和组蛋白Ｈ１０；另外还有人类补体因子Ｂ及类转运ＲＮＡ合成酶的基因。这些基因大多具有相当于抑瘤基因的功能。从而进一步说明鼻咽癌的发生是多基因相互作用的结果。
In order to search the tumor suppressor genes correlated with pathogenesis of human nasopharyngeal carcinoma(NPC), we applied the PCR based subtractive hybridization technique of representational difference analysis(RDA) to the primary culture cells of normal human nasopharyngeal epithelial and HNE1, a poorly differentiated NPC cell line. Following four successive subtractions of HNE1 complementary DNA from normal human nasopharyngeal epithelial cells complementary DNA, difference products were cloned into pGEM T easy vector and nucleotide sequences determined. Comparison of cDNA sequences against the databases indentified 9 known genes. Known genes included TRIP1(TGF β recepor interracting protein), TAF, ezrin, MHCⅡ, actinin, Histone H1 0, Cytokeratin 13, Squalene Synthetase and RNA Synthetase like. Some of them have an effective suppressive ability on the tumor. In this study, we have demonstrated that cDNA RDA is an effective strategy for systematically isolating differences in gene expression between two related but functionally distinct cells. Our results also indicated that the NPC includes interaction of multigenes and this experiment offers a new route for NPC research.
出处
《湖南医科大学学报》
CSCD
1999年第2期103-106,共4页
Bulletin of Hunan Medical University
基金
国家高技术计划资助
湖南省科委资助
关键词
鼻咽肿瘤
抑瘤基因
cDNA-RDA
基因克隆
nasopharyngeal neoplasm
carcinoma
cDNA representational difference analysis *
tumor suppressor gene
