摘要
目的观察维甲酸对大肠粘膜细胞增殖力学变化的影响。方法应用维甲酸对大鼠大肠癌的诱发过程进行干预治疗，观察癌变率及增殖细胞核抗原（ＰＣＮＡ）表达。结果维甲酸治疗组（Ⅱ组）较未加维甲酸治疗的对照组（Ⅰ组）大肠癌发生时间晚且发生率显著降低（Ｐ＜００５）。Ⅰ、Ⅱ组ＰＣＮＡ指数显著高于未用诱癌剂的Ⅲ、Ⅳ组（Ｐ＜００１）。组内对比结果显示，Ⅰ组ＰＣＮＡ表达有随着诱癌时间延长而增强的趋势（Ｐ＜００５），至诱癌后期ＰＣＮＡ指数已达１６８±１４，接近高分化腺癌水平，低于低分化腺癌。Ⅱ、Ⅲ、Ⅳ组内比较差异无显著意义。Ⅱ组ＰＣＮＡ指数早期与Ⅰ组差异无显著意义。中后期显著低于Ⅰ组（Ｐ＜００１）。结论我们认为维甲酸可完全或部分阻逆实验性大肠癌的癌变过程，降低其发生率，为临床应用维甲酸防治大肠癌提供了理论资料。
Objective To study the interference of tretinoic acid (RA) on cell proliferation kinetics of the colorectal mucosa cells. Method We applied tretinoic acid (RA) to treat rat colorectal cancer induced by dimethylhydrazine (DMH) The expression of proliferating cell nuclear antigen(PCNA) was studied. Results It was showed that the incidence of colorectal cancer was significantly lower in RA treated group (group Ⅱ) than in untreated group (group Ⅰ)( P <0 05).The PCNA indices in groupsⅠ,and Ⅱ were significantly higher than in groups Ⅲ and Ⅳ in which carcinogen was not given ( P <0 01).The results also showed that there was a tendency in group Ⅰ that the longer of the stage induced by DMH,the higher of the PCNA index.But there were not significant differences in group Ⅱ,Ⅲ and Ⅳ ( P >0 05).In the early stage there were no significant differences of the PCNA indices between group Ⅰ and group Ⅱ.But after the early stage the PCNA indices in group Ⅱ were significantly lower than in group Ⅰ( P <0 01). Conclution It is therefore concluded that RA inhibits the effect of DMH and decreases the incidence of colon cancer entirely or partially.
出处
《中华普通外科杂志》
CSCD
1999年第2期136-138,共3页
Chinese Journal of General Surgery
关键词
维甲酸
增殖细胞核抗原
大肠肿瘤
抗原
Tretinoin Colorectal neoplasms Proliferating cell nuclear antigen
