摘要
目的：建立普罗帕酮中毒快速检验方法。方法：血清２．５ｍｌ，８ｍｌ乙醚分两次萃取，点于ＨＳＧＦ２５４板上，正庚烷∶三氯甲烷∶无水乙醇∶乙酸乙酯∶甲醇（５∶２∶２．５∶１．５∶２．５）展开５～７ｃｍ，薄层色谱扫描定性和定量检测。结果：血清中普罗帕酮线性检测范围为０．１～６μｇ·ｍｌ－１，最小检出浓度为０．０５μｇ·ｍｌ－１。结论：本法快速、简便、定性定量准确。
OBJECTIVE:To develop a rapid examination for propafenone poisoning.METHODS:Serum(2.5ml)was extracted with 4ml ether twice. HSGF 254 plate was used as the TLC plate, and the plate was developed with n heptane∶trichloromethane∶ethanol∶acetic ether∶methanol(5∶2∶ 2.5 ∶ 1.5 ∶ 2.5 )for 5～7 cm, the qualitative and quantitative analysis of propafenone was carried out by TLC scanning.RESULTS:The extraction curves of propafenone in serum was linear over the range of 0.1 to 6 μg·ml -1 . The limit of detection was ￣ 0.05 μg·ml -1 . CONCLUSIONS:This analysis is simpler, more sensitive, more practised, it is good for propafenone overdosing and poisoning identification.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第4期195-197,共3页
Chinese Journal of Hospital Pharmacy
