摘要
为了解广西鸭圆环病毒(duck circovirus,DuCV)感染情况,根据已发表的鸭圆环病毒序列合成了1对PCR引物,利用该引物对采集于百色、柳州和桂林3个市的321份鸭组织样品进行了检测。结果表明,58份样品能扩增出228bp的特异条带,总阳性率为18.07%;且3个市均能检出阳性样品,阳性率分别为18%(54/300)、5.6%(1/18)和100%(3/3)。结果证实,广西百色、柳州、桂林地区的鸭群中不同程度地存在鸭圆环病毒感染现象。
To investigate the epidemic situation of duck cirovirus（DuCV）in Guangxi,321duck tissue samples collected from Baise,Liuzhou and Guilin cities of Guangxi province were detected by PCR.Results showed that the specific fragments of 228bp were amplified from 58samples,with a total positive rate of 18.07%.Furthermore,positive samples could be found in all 3cities.And positive rate of each cities were 18%（54/300）,5.6%（1/18）and 100%（3/3）,respectively.This investigation demonstrated that the existence of DuCV infection with different levels in duck populations from vary regions of Guangxi province.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第11期156-158,共3页
China Animal Husbandry & Veterinary Medicine
基金
广西科技创新能力与条件建设项目(08-05-01D)
