摘要
探讨RhoE对胃癌转移的影响及可能的机制。用Western Blot(WB)和免疫组织化学检测RhoE蛋白在胃癌组织中的表达;WB,半定量PCR检测RhoE蛋白和mRNA在转移潜能不同的胃癌细胞系中的表达;RhoE的正义表达载体稳定转染胃癌细胞系SGC7901。通过迁移实验、侵袭实验检测其对胃癌细胞体外迁移、侵袭能力的影响。WB检测改变胃癌细胞中RhoE表达后基质金属蛋白酶(MMP)表达变化。结果RhoE蛋白在胃癌组织和细胞系中的表达与胃癌转移相关联;上调RhoE表达能促进胃癌细胞的体外迁移能力和侵袭能力MMP9表达也随之上调。实验结果率先证实RhoE能够促进胃癌转移,这一作用可能是通过上调MMP9实现的。
To investigate the function of RhoE in metastasis of human gastric cancer and possible mechanisms,Immunohistochemistry and Western blot（WB） were used to analyze the protein level of RhoE in gastric cancer tissues.Protein and mRNA of RhoE were separately examined by Western blot and semi-quantitative PCR in two human gastric cancer lines with different metastatic potentials.Plasmid encoding RhoE and empty vector were transfected into SGC7901 cells through stable transfection.Wound-healing assay and invasion assay were used to examine the in vitro migratory and invasive abilities of the transfected cells and control cells.Western blot analysis was performed to examine the changes of MMPs.It is resuledted that RhoE expression is highly related to the metastasis of gastric cancer.Upregulation of RhoE significantly enhances cell migratory and invasive abilities in vitro,and upregulates MMP9 rather than MMP2.The result is that RhoE could enhance the metastasis of gastric cancer through upregulating MMP9.
出处
《科学技术与工程》
2010年第31期7631-7636,共6页
Science Technology and Engineering
基金
国家重点基础研究发展计划(973计划)(2009CB521700)资助
关键词
RHOE
细胞迁移
细胞侵袭
肿瘤转移
胃癌
RhoE cell migration cell invasion tumor metastasis gastric cancer
