摘要
目的:探讨免疫组织化学染色在食管梭形细胞肿瘤鉴别诊断及明确肿瘤的来源的意义。方法:应用HE染色及免疫组织化学染色法,检测18例食管梭形细胞肿瘤标本CD117、CD34、SMA、Desmin和S-100抗体的表达。结果:18例食管梭形细胞肿瘤中CD34、S-100、Desmin的阳性/阴性表达比例分别为4/14、0/18和12/6。CD117:6例阳性,其中2例局部胞膜阳性细胞,4例梭形细胞胞质阳性;12例阴性。SMA:17例肿瘤细胞呈程度不等的弥漫阳性。18例食管梭形细胞肿瘤中12例为平滑肌瘤,6例为间质瘤。结论:免疫组织化学技术能比较准确地判断食管梭形细胞肿瘤的生物学行为和预后,为术后合理选择分子靶向治疗方案提供方向。
Objective To investigate the significance of immunohistochemical method in differential diagnosis and definiting the origin of esophageal spindle cell tumors.Methods Eighteen cases of esophageal stromal turmors were studied by HE staning and immunohistochemistry to detect the expressions of CD117,CD34,SMA,Desmin and S-100.Results In 18cases of esophageal spindle cell tumors,the CD34,S-100,Desmin positive/negative expression rates were 4/14,0/18and 12/6,respectively.6cases of esophageal spindle cell tumors were positive for CD117,among them 2cases were local positive in membrane and 4cases were positive in cytoplasm of spindle cells. 12cases of esophageal spindle cell tumors were negative for CD117.17cases tumor cells were varying degrees diffusely and positive expression for SMA.Among 18cases of esophageal spindle cell tumors,12cases were leiomyoma and 6cases were gastrointestinal stromal tumors.Conclusion Immunohistochemistry could accurately determine the biological behavior and prognosis of esophageal spindle cell tumors,it could provide direction for reasonable choice of molecular target therapy after operation.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期962-964,F0003,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200505220)
关键词
免疫组织化学染色
食管肿瘤
梭形细胞
immunohistochemical staining
esophageal neoplasms
spindle cell
